7. Literaturverzeichnis

Aaronson S (1991) Growth factors and cancer. Science 254:1146-1153

Adams JM, Cory S (1991) Transgenic models of tumor development. Science 254:1161-1167

Aggerholm A, Guldberg P, Hokland M et al. (1999) Extensive intra- and interindividual heterogenity of p15INK4B methylation in acute myeloid leukemia. Cancer Res 59:436-441

Ahuja N, Movan AL, Li Q et al. (1997) Association between CpG island methylation and microsatellite instability in colorectal cancer. Cancer Res 57:3370-3374

Albers B, et al. (1995) Molecular biology of the cell. 3rd edn., Garland Publishing, New York

Antequera F, Boyes J and Bird AP (1990) High levels of de novo methylation and altered chromatin structure at CpG islands in cell lines. Cell 62:503-514

Antillon-Klussmann F, Garcia-Delgado M, Villa-Elizaga I et al. (1995) Mutational activation of ras genes is absent in pediatric osteosarcoma. Cancer Genet Cytogenet 79(1):49-53

Baldini N, Scotlandi K, Barbanti-Brodano G et al. (1995) Expression of P-glycoprotein in high-grade osteosarcomas in relation to clinical outcome. N Engl J Med 333(21):1380-1385

Barletta JM, Rainier S and Feinberg AP (1997) Reversal of loss of imprinting in tumor cells by 5-aza-2`-deoxycitidine. Cancer Res 57:48-50

Bartek J, Bartkova J, Lukas J (1996) The retinoblastoma protein pathway and the restriction point. Curr Opin Cell Biol 8:805-814

Baylin SB, Herman JG, Graff JR et al. (1998) Alterations in DNA methylation: a fundamental aspect of neoplasia. Adv Cancer Res 72:141-196

Belinsky SA, Nikula KJ, Baylin SB et al. (1996) Increased cytosine DNA-methyltransferase activity is target-cell specific and an early event in lung cancer. Proc Natl Acad Sci USA 93:4045-4050

Belka C, Greger V, Zabel B et al. (1991) No evidence for sequences structurally related to the RB1 gene in the human genome. Hum Genet 86:401-403

Bender CM, Pao MM, Jones AP (1998) Inhibition of DNA methylation by 5-Aza-2`-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res 58:95-101

Benedict WF, Yuen-Kai T, Fung P et al. (1988) The gene responsible for the development of retinoblastoma and osteosarcoma. Cancer: 62:1691-1694

Bielack et al. (1996) Cooperative Osteosarkomstudie COSS-96, Therapieprotokoll der Gesellschaft für Pädiatrische Onkologie und Hämatologie

Bieling P, Rehan N, Winkler P et al. (1996) Tumor size and prognosis in aggressively treated osteosarcoma. J Clin Oncol 14(3):848-858

Bird AP and Southern EM (1978) Use of restriction enzymes to study eukaryotic DNA methylation. J Mol Biol 118:27-47

Bishop JM (1987) The molecular genetics of cancer. Science 235:305-311

Bissonette RP, Echeverri F, Mahboubi A et al. (1992) Apoptotic cell death by c-myc is inhibited by bcl-2. Nature 359:552-554

Bookstein R, Shew JY, Chen PL et al. (1990) Suppression of tumorigenicity of human prostate carcinoma cells by replacing a mutated RB gene. Science 247:712-715

Boyes J and Bird A (1992) Repression of genes by DNA methylation depends on CpG density and promoter strength: evidence for involvement of a methyl-CpG binding protein. EMBO J 11:327-333

Cairns J (1975) Mutation selection and the natural history of cancer. Nature 255:197-200

Cantley LC, Auger KR, Carpenter C et al. (1991) Oncogenes and signal transduction. Cell 64:281-302

Cavenee WK, Dryja TP, Philips RA et al. (1983) Expression of recessive alleles by chromosomal mechanisms in retinoblastoma. Nature 305:779-784

Chang MW, Barr E, Seltzer J et al. (1995) Cytostatic gene therapy for vascular proliferative disorders with a constitutively active form of the retinoblastoma gene product. Science 267:518-522

Chen-JX; Zheng-Y; West-M; Tang-MS (1998) Carcinogens preferentially bind at methylated CpG in the p53 mutational hot spots. Cancer-Res.58(10): 2070-5

Chen RZ, Petterson U, Beard C et al. (1998) DNA hypomethylation leads to elevated mutation rates. Nature 395: 89-93

Clark SJ, Harrison J and Frommer M (1995) CpG methylation in mammalian cells. Nature Genet 10:20-27

Clark SJ, Harrison J and Molloy PL (1997) Sp1 binding is inhibited by CpG methylation. Gene 195:67-71

Copeland NE, Hanke CW, Michalak JA (1997) The molecular basis of xeroderma pigmentosum. Dermatol Surg 23(6):447-455

Cryns VL, Thor A, Xu HJ et al (1994) Loss of the retinoblastoma tumor-suppressor gene in parathyroid carcinoma. N Engl J Med 330:757-761

Dahlin DC, Coventry MB (1967) Osteosarcoma: a study of six hundred cases. J Bone Joint Surg 49a:101-110

Dahlin DC, Unni KK (1986) Bone Tumors: general aspects and data on 8542 cases. 4th edn., Charles C. Thomas Publishers, Springfield Il.

Dalla Favera et al. (1982) Human c-myc oncogene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc Natl Acad Sci USA 79:7824-7827

Davis AM, Bell RS, Goodwin PJ (1994) Prognostic factors in osteosarcoma: a critical review. J Clin Oncol 12:423-431

Derrane M (1999) Gene imprinting : making an impression on cancer research. J Natl Cancer Inst 91(1):16-18

Dickmann PS, Barmada M, Gollin SM et al. (1997) Malignancy after retinoblastoma: secondary cancer or recurrence? Hum Pathol 28(2):200-205

Dixon SC, Soriano BJ, Lush RM et al (1997) Apoptosis: its role in the development of malignancies and its potential as a novel therapeutic target. Ann Pharmacother 31(1):76-82

Donehower LA, Harvey M, Slagle B et al. (1992) Mice deficient for p53 are develop-mentally normal but susceptible to spontaneous tumors. Nature 356:215-221

Downward J (1984) Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences. Nature 307:521-527

Dryja TP, Rapaport JM, Weichselbaum R et al. (1984) Chromosome 13 restriction fragment length polymorphism. Hum Genet 65:320-324

Dryja TP, Rapaport JM, Joyce JM (1986) Molecular detection of deletions involving band q14 of chromosome 13 in retinoblastoma. Proc Natl Acad Sci USA 83:7391-7394

el-Deiry WS, Nelkin BD, Celano P et al. (1991) High expression of the DNA methyltransferase gene characterizes human neoplastic cells and progression state of colon cancer. Proc Natl Acad Sci USA 88:3470-3474

Eliyahu D, Michalovitz D, Eliyahu S et al. (1989) Wild-type p53 can inhibit oncogene-mediated focus formation. Proc Natl Acad Sci USA 86:8763-8767

Esteller M, Sanchez M, Rosell R et al. (1999) Detection of aberrant hypermethylation of tumor suppressor genes in serum DNA from non-small cell lung cancer patients. Cancer Res 59.67-70

Fanidi A, Harrington EA, Evan GI (1992) Cooperative interaction between c-myc and bcl2 proto-oncogenes. Nature 359:554-556

Fearon ER, Hamilton SR, Vogelstein B (1987) Clonal analysis of human colorectal cancer Science 238:193-197

Feinberg AP and Vogelstein B (1983) Hypomethylation distinguishes genes of some human cancers from their normal counterparts. Nature 301:89-92

Feinberg AP and Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specifity activity. Anal Biochem 132:6-13

Feinberg AP, Gehrke C, Kuo KC et al. (1988) Reduced genomic 5-methylcytosine content in human colonic neoplasia. Cancer Res 48:1159-1161

Feinberg AP (1993) Genomic imprinting and gene activation in cancer. Nature Genet 4:110-113

Feugeas O, Guriec N, Babin-Boilletot A et al. (1996) Loss of Heterozygosity of the RB gene is a poor prognostic factor in patients with osteosarcoma. J Clin Oncol 14(2):467-472

Fialkow PJ (1976) Clonal origin of human tumors. Biochim Biophys Acta 458:283-321

Ford JM, Hansen PL (1997) Role of DNA excision repair gene defects in the etiology of cancer. Curr Top Microbiol Immunol 221:47-70

Freije JM, MacDonald NJ, Steeg PS (1996) Differential gene expression in tumor metastasis: Nm 23. Curr Top Microbiol Immunol 213(2):215-232

Friend SH, Bernards R, Rogelj S et al. (1986) A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma. Nature 323:643-646

Frommer M, McDonald LE, Millar DS et al. (1992) A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proc Natl Acad Sci USA 89: 1827-1831

Fuchs N, Winkler K (1993) Osteosarcoma. Curr Opin Oncol 5:667-671

Fung YT, Murphree AL, T`Ang A et al. (1987) Structural evidence for the authenticity of the human retinoblastoma gene. Science 236:1657-1661

Glasser DB, Lane JM, Huvos AG et al. (1992) Survival, prognosis and therapeutic response in osteogenic sarcoma. Cancer 69:698-708

Godbout R, Dryja T, Squire J et al. (1983) Somatic inactivation of genes on chromosome 13 is a common event in both hereditary and nonhereditary retinoblastoma tumors. Nature 304:451-453

Gonzalgo M and Jones PA (1997) Mutagenic and epigenetic effects of DNA methylation. Mutat Res 386:107-118

Gonzalgo ML, Liang G, Spruck CH et al. (1997) Identification and characterization of differentially methylated regions of genomic DNA by methylation-sensitive arbitrarily primed PCR. Cancer Res 57:594-599

Gonzalgo ML, Hayashida T, Bender CM et al. (1998) The role of DNA methylation in expression of the p19/p16 locus in human bladder cancer cell lines. Cancer Res 58:1245-1252

Goodrich DW, Lee WH (1993) Molecular characterization of the retinoblastoma susceptibility gene. Biochim Biophys Acta 1155:43-61

Greenblat MS, Benett WP, Hollstein M et al. (1994) Mutations in the p53 tumor suppressor gene: clues to cancer etiology and molecular pathogenesis. Cancer Res.54:4855-4878

Greger V, Passarge E, Höpping W et al. (1989) Epigenetic changes may contribute to the formation and spontaneous regression of retinoblastoma. Hum Genet 83:155-158

Greger V, Debus N, Lohmann D et al. (1994) Frequency and parental origin of hypermethylated RB1 alleles in retinoblastoma. Hum Genet 94:491-496

Greider CW, Blackburn EH (1996) Telomeres, telomerase and cancer. Sci Am 274(2):92-97

Hansen MF, Koufus A, Gallie BL et al. (1985) Osteosarcoma and retinoblastoma: a shared chromosomal mechanism revealing recessive predisposition. Proc Natl Acad Sci USA 82:6216-6220

Harrington EA, Bruce JL, Harlow E et al. (1998) pRB plays an essential role in cell cycle arrest induced by DNA damage. Proc Natl Acad Sci USA 95(20):11945-50

Harris H, Miller O, Klein G et al (1969) Suppression of malignancy by cell fusion. Nature 223:363-368

Harris CC and Hollstein M (1993) Clinical implications of the p53 tumor-suppressor gene. N Engl J Med 329:1318-1327

Hayashi N, Ido E, Ohtsuki Y et al. (1999) An experimental application of gene therapy for human retinoblastoma. Invest Ophtalmol Vis Sci 40(2):265-272

Herman JG, Latif F, Wenig Y et al. (1994) Silencing of the VHL-tumor suppressor gene by DNA methylation in renal carcinoma. Proc Natl Acad Sci USA 91:9700-9704

Herman JG, Graff JR, Myöhänen S et al. (1996) Methylation-specifiv PCR: a novel PCR essay for methylation status of CpG islands. Proc Natl Acad Sci USA 93: 9821-9826

Herman-JG; Umar-A; Polyak-K et al. (1998) Incidence and functional consequences of hMLH1 promoter hypermethylation in colorectal carcinoma. Proc-Natl-Acad-Sci-U-S-A. 95: 6870-6875

Horstmann MA, Pösl M, Scholz RB et al. (1997) Frequent reduction or loss of DCC gene expression in human osteosarcoma. Br J Cancer 75(9):1309-1317

Hsieh CL (1994) Dependence of transcriptional repression on CpG methylation density. Mol Cell Biol 14:5487-5494

Huang HJ, Yee JK, Shew JY et al. (1988) Suppression of the neoplastic phenotype by replacement of the RB gene in human cancer cells. Science 242:1563-1566

Hunter T (1991) Cooperation between oncogenes. Cell 64:249-270

Ishikawa J, Xu HJ, Yandell DW et al. (1991) Inactivation of the retinoblastoma gene in human bladder and renal cell carcinomas. Cancer Res 51:5736-5743

Issa JP, Ottaviano YL, Celano P et al (1994) Methylation of the estrogen receptor CpG island links aging and neoplasia in human colon. Nature Genet 7:536-540

Issa JP, Baylin SB and Herman JG (1997) DNA methylation changes in hematologic malignancies: biologic and clinical implications. Leukemia 1997 Suppl 1:S7-S11

Jaffe N, Frei E, Traggis D (1974) Adjuvant methotrexate and citrovorum-factor treatment of osteogenic sarcoma. N Engl J Med 291:994-997

Jacks T, Fazei A, Schmitt EM et al. (1992) Effects of an RB mutation in the mouse. Nature 359:295-300

Jarrard DF, Kinoshita H, Shi Y (1998) Methylation of the androgen receptor promoter CpG island is associated with loss of androgen receptor expression in prostate cancer cells. Cancer Res 58(23):5310-14

Jones PA and Gonzalgo ML (1997) Altered DNA methylation and genome instability: a new pathway to cancer? Proc Natl Acad Sci 94:2103-2105

Jones PA, Wolkowicz MJ, Rideout WM et al. (1990) De novo methylation of the MyoD1 CpG island during the establishment of immortal cell lines. Proc Natl Acad Sci USA 87:6117-6121

Jones PA (1996) DNA methylation errors and cancer. Cancer Res 56:2463-2467

Kane MF, Loda M, Gaida GM et al (1997) Methylation of the hMLH1 promoter correlates with lack of expression of hMLH1 in sporadic colon tumors and mismatch repair-defective human tumor cell lines. Cancer Res 57(5):808-811

Keohavong P, Zhu D, Whiteside TL et al. (1997) Detection of infrequent and multiple K-ras mutations in human tumors and tumor-adjavent tissues. Annal Biochem 247(2):394-403

Kiviat N (1996) Natural history of cervical neoplasia: overview and update. Am J Obstet Gynecol 175(4):1099-1104

Klingelhutz AJ (1997) Telomerase activation and cancer. J Mol Med 75(1):45-49

Knudson AG (1971) Mutation and cancer: statistical study of retinoblastoma. Proc Natl Acad Sci USA 68:820-823

Kruzelock RP, Murphy EC, Strong LC et al. (1997) Localization of a novel tumor-suppressor locus on human chromosome 3q important in osteosarcoma tumorigenesis. Cancer Res 57(1):106-109

Ladanyi M, Cha C, Lewis R et al.(1993) MDM2 gene amplification in metastatic osteosarcoma. Cancer Res 53:16-18

Laird PW and Jaenisch R (1994) DNA methylation and cancer. Hum Mol Genet 3:1487-1495

Laird PW, Jacksongrusby L, Fazelli A et al. (1995) Suppression of intestinal neoplasia by DNA hypomethylation. Cell 81:197-205

Lane DP, Crawford LW (1979) T-antigen is bound to a host protein in SV 40-transformed cells. Nature 278:261-263

Lane D (1992) p53, guardian of the genome. Nature 358:15-16

Lee EY, Chang CY, Hu N et al. (1992) Mice deficient for RB are nonviable and show defects in neurogenesis and hematopoiesis. Nature 359:288-294

Lee WH, Bookstein R, Hong F et al. (1987a) Human retinoblastoma susceptibility gene: cloning, identification and sequence. Science 235: 1394-1399

Lee WH, Shew JY, Hong FD et al. (1987b) The retinoblastoma susceptibility gene encodes a nuclear phosphoprotein associated with DNA binding activity. Nature 329:642-645

Lee YW, Klein C, Kargacin B et al (1995) Carcinogenic nickel silences gene expression by chromatin condensation and DNA methylation: a new model for epigenetic carcinogens. Mol Cell Biol 15:2547-2557

Lengauer C, Kinzler KW and Vogelstein B (1997) DNA methylation and genetic instability in colorectal cells. Proc Natl Acad Sci 94:2545-2550

Leung SY, Yuen ST, Chung LP et al. (1999) hMLH1 promoter methylation and lack of hMLH1 expression in sporadic gastric carcinomas with high-frequency microsatellite instability. Cancer Res 59(1):159-164

Li E, Beard C and Jaenisch R (1993) Role for DNA-Methylation in genomic imprinting. Nature 366:362-365

Li FP, Cassady JR, Jaffe N (1975) Risk of second tumor in survivors of childhood cancer. Cancer 35:1230-1235

Li FP, Fraumeni JF (1969) Rhabdomyosarcoma in children: epidemiology study and identification of a familial cancer syndrome. J Natl Cancer Inst 43:1356-1373

Li FP, Fraumeni JF et al. (1988) A cancer family syndrome in twenty-four kindreds. Cancer Res 48:5358-5362

Li H, Bestor T and Jaenisch R (1992) Targeted mutation of the DNA-methyltransferase gene results in embryonic lethality. Cell 69:915-926

Li W, Fan J, Hochhauser D et al. (1995) Lack of functional retinoblastoma protein mediates increased resistance to antimetabolites in human sarcoma cell lines. Proc Natl Acad Sci 92:10436-10440

Linzer DIH, Levine AJ (1979) Characterization of a 54kd cellular SV 40 tumor antigen present in SV 40 transformed cells and uninfected embryonal carcinoma cells. Cell 17:43-52

Lonardo F, Ueda T, Huvos AG et al. (1997) p53 and MDM2 alterations in osteosarcoma: correlation with clinicopathologic features and proliferation rate. Cancer 79(8):1541-1547

Lowy D and Willumsen BM (1993) Function and regulation of Ras. Annu Rev Biochem 62:851-891

Lu Y, Marler DF, Hu SX et al. (1996) Retinoblastoma protein inhibits INF-g induced apoptosis. Oncogene 12:1809-1819

Lynch HT, Smyrk T, Lynch JF (1996) Overview of natural history, pathology, molecular genetics and management of HNPCC (Lynch Syndrome). Int J Cancer 69:38-43

MacLeod AR and Szyf M (1995) Expression of an antisense oligodeoxynucleotide to the DNA methyltransferase mRNA induces DNA demethylation and inhibits tumorigenesis. J Biol Chem 270:8037-8043

Makos-Wales M, Biel M, El Deiry W et al. (1995) p53 activates expression of HIC-1, a new candidate tumor suppressor gene on 17p13.3 Nature Med 1:570-577

Mancini D, Singh S, Ainsworth P et al. (1997) Constitutively methylated CpG Dinucleotides as mutation hot spots in the retinoblastoma gene (RB1). Am J Hum Genet 61:80-87

Masuda H, Miller C, Koeffler HP et al. (1987) Rearrangement of the p53 gene in human osteogenic sarcoma. Proc Natl Acad Sci USA 84:7716-7719

McGee TL, Yandell DW, Dryja TP (1989) Structure and partial genomic sequence of the human retinoblastoma susceptibility gene. Gene 80: 119-128

Meadows A, Strong LC, Li FP et al. (1980) Bone sarcoma as a second malignant neoplasm in children. Cancer: 46:2603-2606

Meinkoth J and Wahl G (1984) Hybridization of nucleic acids immobilized on solid supports. Anal Biochem 138-267-284

Merlo A, Herman J, Mao L et al. (1995) 5`-CpG-island methylation is associated with transcriptional silencing of the tumor suppressor p16/CDKN2/MTSI in human cancers. Nature Med 1:686-692

Meyers PA, Heller G, Healey JH et al. (1993) Osteogenic sarcoma with clinically detectable metastasis at initial presentation. J Clin Oncol 11(3):449-453

Migeon BR (1994) X-chromosome inactivation: molecular mechanisms and genetic consequence. Trends Genet 10:230-235

Miller CW, Aslo A, Tsay C et al. (1990) Frequency and structure of p53 rearrangements in human osteosarcomas. Cancer Res 50:7950-7954

Mullis K, Faloona F, Scharf S et al. (1986) Specific enzymatic amplification of DNA in vitro: The polymerase chain reaction. Cold Spring Harbor Symp Quant Biol 51:263-273

Nasmyth K (1996) Retinoblastoma protein: another role rolls in. Nature 382:28-29

Nowell PC (1976) The clonal evolution of tumor cell populations. Science 194:23-28

Nyce JW (1997) Drug-induced DNA hypermethylation: a potential mediator of acquired drug resistance during cancer chemotherapy. Mutat Res 386:153-161

Ohtani-Fujita N, Dryja T, Rapaport JM et al. (1997) Hypermethylation in the retinoblastoma gene is associated with unilateral, sporadic retinoblastoma. Cancer Genet Cytogenet 98:43-49

Ohtani-Fujita N, Fujita T, Aoike A et al. (1993) CpG methylation inactivates the promoter activity of the human retinoblastoma tumor-suppressor gene. Oncogene 8:1063-1067

Oliner JD, Kinzler KW, Meltzer PS et al. (1992) Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358:80-83

Onda M, Matsuda S, Higahi S et al. (1996) Erb-2 expression is correlated with poor prognosis for patients with osteosarcoma. Cancer 77:71-78

O`Reilly MS, Holmgren L, Chen CC et al. (1996) Angiostatin induces and sustains dormancy of human primary tumors in mice. Nature Med 2:689-692

O`Reilly MS; Boehm T, Shing Y et al. (1997) Endostatin: an endogenous inhibitor of angiogenesis and tumor growth. Cell 88:277-285

Ottaviano YL, Issa J, Park F et al. (1994) Methylation of the estrogen receptor gene CpG island marks loss of estrogen receptor expression in human breast cancer cells. Cancer Res 54:2552-2555

Ozaki T, Ikeda S, Kawai A, et al. (1993) Alterations of retinoblastoma susceptible gene accompanied by c-myc amplification in human bone and soft tissue tumors. Cell Mol Biol 39(2):235-242

Pereira-Smith et al. (1983) Evidence for the recessive nature of cellular immortality. Science 221:964-966

Pinto A and Zagonel V (1993) 5-aza-2`-deoxycytidine and 5-azacytidine in the treatment of acute myeloid leukemias and myelodysplastic syndromes: past, present and future trends. Leukemia 7 (Suppl 1):51-60

Pritchard-Jones K (1996) Genetics of childhood cancer. Brit Med Bull 52(4):704-723

Provisor AJ, Ettinger LJ, Nachman JB et al. (1997) Treatment of nonmetastatic osteosarcoma of the extremity with preoperative and postoperative chemotherapy: a report from the children`s cancer group. J Clin Oncol 15:76-84

Rabbits TH (1994) Chromosomal translocations in human cancer. Nature 373:143-149

Ramchandai S, MacLeod AR, Pinard M, et al. (1997) Inhibition of tumorigenesis by a cytosine-DNA, methyltransferase, antisense oligodeoxynucleotide. Proc Natl Acad Sci USA 94:684-689

Ray JS, Harbison ML, McClain RM et al. (1994) Alterations in the methylation status and expression of the raf oncogene in phenobarbital-induced and spontaneous B6C3F1 mouse liver tumors. Mol Carcinog 9:155-166

Razin A and Kafri T (1994) DNA methylation from embryo to adult. Prog Nucl Acid Res Mol Biol 48:53-81

Rideout W, Coetzee G, Olumi A et al. (1990) 5-methylcytosine as an endogenous mutagen in the human LDL receptor and p53 genes. Science 249:1288-1290

Rideout W, Eversole-Cire P, Spruck CH, et al. (1994) Progressive increases in the methylation status and heterochromatization of the MyoD CpG island during oncogenic transformation. Mol Cell Biol 14:6143-6152

Rowan S, Fisher DE (1997) Mechanisms of apoptic cell death. Leukemia 11(4):457-465

Sakai T, Toguchida J, Ohtani N et al. (1991) Allele-specific hypermethylation of the retinoblastoma tumor-suppressor gene. Am J Hum Genet 48:880-888

Salzer-Kuntschik M, Brand G, Delling G (1983) Bestimmung des morphologischen Regressionsgrades nach Chemotherapie bei malignen Knochentumoren. Pathologie 4:135-141

Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor

Schajowicz F, Sissons HA, Sobin LH (1995) The World Health Organization`s histologic classification of bone tumors: a commentary on the second edition. Cancer 75(5):1208-1214

Schmutte C, Yang AS, Nguyen TT et al. (1996) Mechanisms for the involvement of DNA methylation in colon carcinogenesis. Cancer Res 56:2375-2381

Scholz RB, Kabisch H, Weber B et al. (1992) Studies of the RB1 gene and the p53 gene in human osteosarcomas. Ped Hem Oncol 9:125-137

Schroeder M and Mass MJ (1997) CpG methylation inactivates the transcriptional activity of the promoter of the human p53 tumor suppressor gene. Biochem Biophys Res Commun 235(2):403-406

Schwab M, Elison J, Bush M et al (1984) Enhanced expression of the human gene N-myc consequent to amplification of DNA may contribute to malignant progression of neuroblastoma. Proc Natl Acad Sci USA 81:4940-4944

Scrable H, Cavenee W, Ghavimi F et al. (1989) A model for embryonal rhabdomyosarcoma tumorigenesis that involves genome imprinting. Proc Natl Acad Sci USA 86:7480-7484

Sealey PG and Southern EM (1982) Gel electrophoresis of DNA. In: Rickwood D, Hames BD (eds.): Gel electrophoresis of nucleic acids: a practical approach, IRL Press, Oxford, Washington DC

Seeger RC, Brodeur GM, Sather H et al. (1985) Association of multiple copies of the N-myc oncogene with rapid progression of neuroblastomas. N Engl J Med 313:1111-1116

Sellers WR and Kaelin WG (1997) Role of the retinoblastoma protein in the pathogenesis of human cancer. J Clin Oncol 15:3301-3312

Sherr CJ (1996) Cancer cell cycles. Science 274:1672-1677

Shiozaki H, Oka H, Inove M et al. (1996) E-cadherin mediated adhesion system in cancer cells. Cancer 77(8):1605-1613

Shmookler-Reis RJ and Goldstein S (1982) Interclonal variation in methylation patterns for expressed and non-expressed genes. Nucleic Acids Res 10:4293-4304

Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis). J Mol Biol 98:503-517

Stanbridge E (1976) Suppression of malignancy in human cells. Nature 260:17-20

Stirzacker C, Millar DS, Paul CL et al. (1997) Extensive DNA methylation spanning the RB promoter in retinoblastoma tumors. Cancer Res 57(11):2229-2237

Surani-MA (1998) Imprinting and the initiation of gene silencing in the germ line. Cell 93(3): 309-12

Suzuki H, Veda R, Takahashi T et al. (1994) Altered imprinting in lung cancer. Nature Genet 6:332-333

Szyf M (1996) The DNA methylation machinery as a target for anticancer therapy. Pharmacol Ther 70(1):1-37

T`Ang A, Varley JM, Chakraborty S et al. (1988) Structural rearrangement of the retinoblastoma gene in human breast carcinomas. Science 242:263-266

Taniguchi T, Sullivan MJ, Ogawa O et al. (1995) Epigenetic changes encompassing the IGF2/H19 locus associated with relaxation of IGF2 imprinting and silencing of H19 in Wilms tumor. Proc Natl Acad Sci USA 92:2159-2163

Toguchida J, Ishizaki K, Nakamura Y et al. (1988) Chromosomal reorganization for the expression of recessive mutations of retinoblastoma susceptibility gene in the development of osteosarcoma. Cancer Res 48:3939-3943

Toguchida J, Yamaguchi T, Dayton SH et al. (1992) Prevalence and spectrum of germline mutations of the p53 gene among patients with sarcoma. N Engl J Med 326:1301-1308

Toguchida J, McGee TL, Paterson JC et al. (1993) Complete genomic sequence of the human retinoblastoma susceptibility gene. Genomics 17:535-543

Tornaletti S and Pfeiffer G (1995) Complete and tissue-independent methylation of CpG sites in the p53 gene: implications for mutations in human cancers. Oncogene 10:1493-1499

Turker MS and Bestor TH (1997) Formation of methylation patterns in the mammalian genome. Mutat Res 386(2):119-130

Tycko B (1997) DNA methylation in genomic imprinting. Mutat Res 386:131-140

Uchida A, Myoui A, Araki N et al. (1997) Neoadjuvant chemotherapy for pediatric osteosarcoma patients. Cancer 79:411-415

Uozaki H, Horiuchi H, Ishida T et al. (1997) Overexpression of resistance-related proteins (metallothioneins, glutathione-S-transferase pi, heat shock protein 27, and lung resistance-related protein) in osteosarcoma. Relationship with poor prognosis. Cancer 79(12):2336-2344

Verkaik NS, Trapman J, Romijn JC et al. (1999) Down-regulation of CD44 expression in human prostatic carcinoma cell lines is correlated with DNA hypermethylation. Int J Cancer 80(3):439-443

Vogelstein B, Kinzler KW (1993) The multistep nature of cancer. Trends Genet 9:138-141

Wadayama B, Toguchida J, Shimizu T et al. (1994) Mutation spectrum of the retinoblastoma gene in osteosarcoma. Cancer Res 54:3042-3048

Wagener C (1996) Molekulare Onkologie: Veränderung und Wirkung von Tumorgenen und Tumorproteinen. 1.Auflage, Thieme Verlag, Stuttgart

Wahlfors J, Hiltunen H, Heinonen K et al. (1992) Genomic hypomethylation in human chronic lymphocyte leukemia. Blood 80:2074-2080

Warnecke PM, Stirzacker C, Melki Jr et al. (1997) Detection and measurement of PCR bias in quantitativ methylation analysis of bisulphite-treated DNA. Nucleic Acids Res 25(2):4422-4426

Weinberg RA (1995) The retinoblastoma protein and cell cycle control. Cell 81:323-330

Weinberg RA (1996) How cancer arises. Sci Am 274(2):32-40

White E (1994) p53: guardian of RB. Nature 371:21-22

White RJ, Trouche D, Martin K et al. (1996) Repression of RNA polymerase III transcription by the retinoblastoma protein. Nature 382:88-90

Winkler K, Bielack S, Delling G et al. (1993) Treatment of osteosarcoma: experience of the Cooperative Osteosarcoma Study Group (COSS). Cancer Treat Res 62:269-277

Wong IH, Lo YM, Zhang J et al. (1999) Detection of aberrant p16 methylation in the plasma and serum of liver cancer patients. Cancer Res 59:71-73

Wu J, Issa JP, Hermann J et al. (1993) Expression of an exogenous eukaryotic DNA methyltransferase gene induces transformation of NIH3T3 cells. Proc Natl Acad Sci USA 90:8891-8895

Xu HJ, Hu SX, Cagle PT et al. (1991) Abscence of retinoblastoma protein expression in primary non-small cell lung carcinomas. Cancer Res 51:2735-2739

Xu HJ (1997) Strategies for approaching retinoblastoma tumor suppressor gene therapy. Adv Pharmacol 40:369-397

Yamaguchi T, Toguchida J, Yamamuro T et al. (1992) Allelotype analysis in osteosarcomas: frequent allele loss on 3q, 13q, 17p, and 18q. Cancer Res 52:2419-2423

Zingg JM and Jones PA (1997) Genetic and epigenetic aspects of DNA methylation on genome expression, evolution, mutation and carcinogenesis. Carcinogenesis 18(5):869-882

zur Hausen H (1991) Viruses in human cancer. Science 254:1167-1173