DC ElementWertSprache
dc.contributor.advisorBoettcher, Michael-
dc.contributor.authorHeuer, Annika-
dc.date.accessioned2021-12-21T14:18:40Z-
dc.date.available2021-12-21T14:18:40Z-
dc.date.issued2021-
dc.identifier.urihttps://ediss.sub.uni-hamburg.de/handle/ediss/9389-
dc.description.abstractBackground: Neutrophilic granulocytes (NG) infiltrate tissue after trauma and mediate pro- and anti-inflammatory activities mainly through the formation of neutrophilic extracellular traps (NETs). NETs promote microvascular damage in the zone of stasis leading to impaired healing. We hypothesized that targeting NETs via DNase1 application and peptidyl arginine deminase type IV (PAD4) knock-out, reduces inflammation, promotes reepithelization and reduces scarring in an experimental model of primary and secondary injury. Methods: A cutaneous full-thickness wound was induced via either (1) surgical incision or (2) thermal injury of 93 C57BL6/J mice with 3 different genotypes: wildtype, PAD4-, and DNase1-Knockout (KO). Wildtype animals either received DNase1 or a vehicle via i.p. route. Wound assessment and euthanasia were conducted. Endpoints were macroscopic appearance (scar scale), time until full re-epithelization (H&E staining), scar structure (collagen I/III ratio, fiber organization and density), wound contractility (SMA), as well as activation of NG (NE, MPO) and NETs release (H3cit). Results: DNase1 treatment or PAD4-KO led to a significant improvement of macroscopic scar appearance and faster wound closure, while wound stability and contractibility was not negatively affected.en
dc.language.isomulde_DE
dc.publisherStaats- und Universitätsbibliothek Hamburg Carl von Ossietzkyde
dc.rightshttp://purl.org/coar/access_right/c_abf2de_DE
dc.subjectVerbrennungde
dc.subjectDNasede
dc.subjectAdhäsionende
dc.subjectTherapiede
dc.subjectWundheilungde
dc.subject.ddc610: Medizinde_DE
dc.titleTherapeutic Targeting of Neutrophil Extracellular Traps Improves Primary and Secondary Intention Wound Healing in Miceen
dc.title.alternativeTherapeutische Hemmung der Neutrophilen Extrazellulären Traps Verbessert die Primäre und Sekundäre Wundheilung im Mausmodellde
dc.typedoctoralThesisen
dcterms.dateAccepted2021-12-08-
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/de_DE
dc.rights.rshttp://rightsstatements.org/vocab/InC/1.0/-
dc.subject.gndSierrade_DE
dc.type.casraiDissertation-
dc.type.dinidoctoralThesis-
dc.type.driverdoctoralThesis-
dc.type.statusinfo:eu-repo/semantics/publishedVersionde_DE
dc.type.thesisdoctoralThesisde_DE
tuhh.type.opusDissertation-
thesis.grantor.departmentMedizinde_DE
thesis.grantor.placeHamburg-
thesis.grantor.universityOrInstitutionUniversität Hamburgde_DE
dcterms.DCMITypeText-
datacite.relation.IsSupplementedBy10.3389/fimmu.2021.614347de_DE
dc.identifier.urnurn:nbn:de:gbv:18-ediss-97587-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.creatorGNDHeuer, Annika-
item.languageiso639-1other-
item.creatorOrcidHeuer, Annika-
item.advisorGNDBoettcher, Michael-
Enthalten in den Sammlungen:Elektronische Dissertationen und Habilitationen
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