The role of TRPM4 in the fundamental properties of microglia

Abstract

In a MS mouse model, TRPM4 influences the disease course negatively. Its genetic KO or pharmacological inhibition induces neuroprotective effects. The focus of this study lies on microglial properties and how they are modulated by TRPM4. For this study the TRPM4 antagonists 9-Phenanthrol, Glibenclamide and CBA were used. The assessment of microglia under TRPM4 block by means of 2P microscopy has shown no changes in their motility. However, results of IHC show that their morphology is modelated by the TRPM4, correlating with their transition to an activated, neurodegeneration-promoting state. Their chemotatic response is also hindered by TRPM4 genetic KO. Thus, TRPM4 imposes itself as a key player in modulating microglial dependent inflammatory response and can consequently be a potential target for the control of neurodegenerative processes.