Volltextdatei(en) vorhanden
Titel: An investigation of interaction between Neuroserpin and its putative targets PC1/3, PC2 and Furin with a YFP-protein complementation assay
Sonstige Titel: Eine Untersuchung über die Interaktion zwischen Neuroserpin und seinen vermeintlichen Zielproteasen PC1/3, PC2 und Furin unter Verwendung eines YFP-protein complementation assay
Sprache: Englisch
Autor*in: Wiesmüller, Felix Johannes Alfred
Schlagwörter: neuroserpin; PC1/3; PC2; YFP; YFP-PCA; PCA; tPA; POMC; ACTH; FENIB
GND-Schlagwörter: Furin
Erscheinungsdatum: 2015
Tag der mündlichen Prüfung: 2015-12-07
Zusammenfassung: 
Neuroserpin is a serine protease inhibitor of the central and peripheral nervous system. It is involved in a vast variety of physiologic as well as pathologic pathways. Physiologic functions range from regulation of neuroanatomic properties (e.g., neurogenesis, synaptogenesis and synaptic plasticity) to psychologic properties (e.g., regulation of the emotional state). Neuroserpin is neuroprotective in ischemic brain injury and epilepsy, yet promotes brain metastasis. Most importantly, mutations in the neuroserpin gene lead to a disease called FENIB via accumulation of neuroserpin polymers. While the underlying cause of FENIB is partially understood, the mechanisms by which neuroserpin regulates the forementioned functions are still unknown. In vitro data suggest tPA as a target of neuroserpin. However, tPA-neuroserpin complexes have never been isolated in vivo and tPA-independent roles for neuroserpin have been described.

In this study, it was hypothesized that other targets besides tPA might exist. As a result, the three proteins including PC1/3, PC2 and Furin were investigated as likely targets. Due to the short-lived nature of complexes between neuroserpin and proteases, the YFP-protein fragment complementation assay was performed providing a method that enables detection of even weak or transient interactions between two proteins.

Results from this study revealed YFP-fragment complementation in cotransfections of neuroserpin with PC2, indicating interaction between the proteins. No interaction was observed in cotransfections of neuroserpin with PC1/3 or neuroserpin with Furin. However, a >170kDa neuroserpin-complex by Western blot analysis was observed when cotransfecting neuroserpin and Furin. This strongly suggests that this complex consists of neuroserpin and an unidentified protein, which possibly is activated by Furin. To further analyze the in vivo relationship between neuroserpin and PC2, the protein expression was quantified in pituitary and whole brain homogenates of neuroserpin knock-out and wild-type animals. This showed a tendency toward an inverse correlation between neuroserpin and PC2 protein levels. Follow up research will help to disclose the molecular mechanisms and further understand the relevance of these observations.  
URL: https://ediss.sub.uni-hamburg.de/handle/ediss/6606
URN: urn:nbn:de:gbv:18-77289
Dokumenttyp: Dissertation
Betreuer*in: Glatzel, Markus (Prof. Dr.)
Enthalten in den Sammlungen:Elektronische Dissertationen und Habilitationen

Dateien zu dieser Ressource:
Datei Beschreibung GrößeFormat  
Dissertation.pdf4.04 MBAdobe PDFÖffnen/Anzeigen
Zur Langanzeige

Diese Publikation steht in elektronischer Form im Internet bereit und kann gelesen werden. Über den freien Zugang hinaus wurden durch die Urheberin / den Urheber keine weiteren Rechte eingeräumt. Nutzungshandlungen (wie zum Beispiel der Download, das Bearbeiten, das Weiterverbreiten) sind daher nur im Rahmen der gesetzlichen Erlaubnisse des Urheberrechtsgesetzes (UrhG) erlaubt. Dies gilt für die Publikation sowie für ihre einzelnen Bestandteile, soweit nichts Anderes ausgewiesen ist.

Info

Seitenansichten

34
Letzte Woche
Letzten Monat
geprüft am 16.04.2021

Download(s)

6
Letzte Woche
Letzten Monat
geprüft am 16.04.2021
Werkzeuge

Google ScholarTM

Prüfe